Cell Signaling Phospho-Pp1alpha (Thr320) Antibody

Name: Cell Signaling Phospho-Pp1alpha (Thr320) Antibody
SKU: CSIG-2581S
Price: 360.57 USD

List Price $360.57 Your Price $360.57

Cell Signaling Phospho-Pp1alpha (Thr320) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2581S

MFG.PART: 2581S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	38
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-PP1α (Thr320) Antibody detects PP1α only when phosphorylated at Thr320. This antibody may cross-react with the phosphorylated δ or γ isoforms of PP1.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr320 of human PP1α. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (1). Involvement in cell cycle regulation is one of the biological functions of PP1 (1). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (4).

Specifications