Cell Signaling Gli1 Antibody

Name: Cell Signaling Gli1 Antibody
SKU: CSIG-2553S
Price: 397.94 USD

List Price $397.94 Your Price $397.94

Cell Signaling Gli1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2553S

MFG.PART: 2553S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	160
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	GLI1 Antibody detects endogenous levels of total GLI1 protein.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly420 of human GLI1. Antibodies are purified by protein A and peptide affinity chromatography
Background	GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Kruppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2, and GLI3 (3). These GLI proteins are similar to the Drosophila homolog Cubitus interruptus (Ci) and function as transcription factors activated by the Hedgehog signaling pathway. Hedgehog signaling plays an important role in animal development, and research studies have shown that this pathway is aberrantly activated in many types of cancers (4,5).GLI1 itself is a transcriptional target of the Hedgehog signaling pathway (6-8) and is used as a marker of Hedgehog signaling activation in cancer research (9,10).

Specifications