Cell Signaling Vav1 Antibody

Name: Cell Signaling Vav1 Antibody
SKU: CSIG-2502S
Price: 353.50 USD

List Price $353.50 Your Price $353.50

Cell Signaling Vav1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2502S

MFG.PART: 2502S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	95
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Simple Western™: 1:10 - 1:50, Flow Cytometry (Fixed/Permeabilized): 1:25}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Vav Antibody detects endogenous levels of total Vav1 protein. The antibody does not cross-react with Vav2, Vav3 or CelVav.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr174 of human Vav. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3).

Specifications