Cell Signaling Eif5 Antibody

Name: Cell Signaling Eif5 Antibody
SKU: CSIG-2480S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Eif5 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2480S

MFG.PART: 2480S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	58
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	eIF5 Antibody detects endogenous levels of total eIF5 protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence surrounding Val417 of human eIF5. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Eukaryotic translation initiation factor 5 (eIF5) is crucial for the assembly of translation initiation complex and plays an important role in protein synthesis (1). eIF5 interacts with the 43S initiation complex to stimulate hydrolysis of GTP bound to eIF2 (1-3). Studies suggest that eIF5 functions as the GTPase-activating protein (GAP) in the hydrolysis of GTP-bound eIF2 (4,5). This hydrolysis leads to the release of initiation factors from the 40S ribosomal subunit, which is a necessary step in the formation of the 80S initiation complex (1).

Specifications