Cell Signaling Eea1 Antibody

Name: Cell Signaling Eea1 Antibody
SKU: CSIG-2411S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Eea1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2411S

MFG.PART: 2411S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	170
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:25, Immunofluorescence (Immunocytochemistry): 1:100}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	EEA1 Antibody detects endogenous levels of total EEA1 protein. Nucleolar background has been observed in some cell lines by immunofluorescence.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly14 of human EEA1 protein. Antibodies are purified by peptide affinity chromatography.
Background	EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7).

Specifications