Cell Signaling Wnt3a Antibody

Name: Cell Signaling Wnt3a Antibody
SKU: CSIG-2391S
Price: 397.94 USD

List Price $397.94 Your Price $397.94

Cell Signaling Wnt3a Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2391S

MFG.PART: 2391S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	M
Sensitivity	Transfected Only
Molecular Weight (kDa)	42
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Wnt3a antibody detects transfected Wnt3a protein in L cells and the conditioned medium from L/Wnt3a cells. Based on homology, it is expected to also recognize Wnt3.
Species Reactivity	Mouse
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp294 of human Wnt3a. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The Wnt family includes several secreted glycoproteins that play important roles in animal development (1). There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins (2). Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathways (3). The canonical Wnt/β-catenin pathway also requires a coreceptor from the low-density lipoprotein receptor family (4). Aberrant activation of Wnt signaling pathways is involved in several types of cancers (5).Wnt3a protein is palmitoylated and can function as a growth factor for hematopoietic stem cells (6). Although functionally distinct, Wnt3a shows high homology to Wnt3 (7).

Specifications