Cell Signaling Villin-1 (R814) Antibody

Name: Cell Signaling Villin-1 (R814) Antibody
SKU: CSIG-2369S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Villin-1 (R814) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2369S

MFG.PART: 2369S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	95
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Villin-1 (R814) Antibody recognizes endogenous levels of total villin-1 protein.
Species Reactivity	Human, Mouse
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human villin-1. Antibodies are purified using protein A and peptide affinitty chromatography.
Background	Villin is a member of the gelsolin family of calcium-regulated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli (1,2). Phosphorylation of villin at Tyr60, 81, and 256 may be involved in the regulation of cell migration (3). Expression of villin is increased in colorectal cancers (4), and villin-1 function appears to be involved in progressive cholestasis and hepatic failure (5).

Specifications