Cell Signaling Phospho-Tbk1/Nak (Ser172) (D52c2) Xp ® Rabbit mAb (Alexa Fluor ® 532 Conjugate)

Name: Cell Signaling Phospho-Tbk1/Nak (Ser172) (D52c2) Xp ® Rabbit mAb (Alexa Fluor ® 532 Conjugate)
SKU: CSIG-20399S
Price: 481.77 USD

List Price $481.77 Your Price $481.77

Cell Signaling Phospho-Tbk1/Nak (Ser172) (D52c2) Xp ® Rabbit mAb (Alexa Fluor ® 532 Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-20399S

MFG.PART: 20399S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl (50 tests)
Reactivity	H M
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (Alexa Fluor® 532 Conjugate) detects endogenous levels of TBK1 only when phosphorylated at Ser172. This antibody may cross-react with phospho-IKKε.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser172 of human TBK1.
Background	TBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK, and found to function upstream of NIK and IKK in the activation of NF-κB (2). TBK1 induces IκB degradation and NF-κB activity through IKKβ. TBK1 may mediate IKK and NF-κB activation in response to growth factors that stimulate PKCε activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3).TBK1 is phosphorylated at Ser172 within its activation loop, which is necessary for its kinase activity including the downstream phosphorylation of IRF3 (4,5).

Specifications