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Cell Signaling Myc-Tag (E4f4d) Mouse mAb
List Price
$342.39
Your Price
$342.39
Cell Signaling Myc-Tag (E4f4d) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-20229S
MFG.PART:
20229S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | All |
| Sensitivity | Transfected Only |
| Source/Isotype | Mouse IgG2b |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Myc-Tag (E4F4D) Mouse mAb detects detects exogenously expressed proteins containing the Myc epitope tag. This antibody recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Myc-Tag (E4F4D) Mouse mAb detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated.The antibody may cross-react with c-myc protein. The antibody may weakly cross-react with a protein of unknown origin at ~90kDa. Low level cross-reactivity was observed using secondary antibodies targeting mouse IgG3, but not mouse IgG1 or IgG2a, by immunofluorescence when used at concentrations above the recommended range. |
| Species Reactivity | All Species Expected |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc protein (EQKLISEEDL). |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1). |
| SKU | CSIG-20229S |
|---|---|
| Featured | No |
| Supplier Part Number | 20229S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |