Cell Signaling Il-6ralpha/Cd126 (E5b3p) Rabbit mAb

Name: Cell Signaling Il-6ralpha/Cd126 (E5b3p) Rabbit mAb
SKU: CSIG-18935S
Price: 342.39 USD

List Price $342.39 Your Price $342.39

Cell Signaling Il-6ralpha/Cd126 (E5b3p) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-18935S

MFG.PART: 18935S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	70-100
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:50 - 1:200, Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400, Flow Cytometry (Live): 1:100 - 1:400}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	IL-6Rα/CD126 (E5B3P) Rabbit mAb recognizes endogenous levels of total IL-6Rα/CD126 protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human IL-6Rα/CD126 protein.
Background	The IL-6 receptor is a heterodimeric complex that consists of a ligand-binding IL-6 receptor α (IL-6Rα) subunit and a signaling component, gp130 (1). Binding of IL-6 to IL-6Rα results in receptor dimerization with gp130 and subsequent STAT3 activation (1). IL-6Rα is cleaved from the cell surface by ADAM17 (1,2). In humans, soluble IL-6Rα is also generated via alternatively spliced mRNA (1,3). Soluble IL-6Rα binds to IL-6 and can stimulate signaling via membrane bound gp130 in a process known as “trans-signaling” (1). It is through trans-signaling that IL-6 stimulates cells that do not express membrane bound IL-6Rα (1).

Specifications