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Cell Signaling Cdyl (E7s8t) Rabbit mAb
List Price
$342.39
Your Price
$342.39
Cell Signaling Cdyl (E7s8t) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-18764S
MFG.PART:
18764S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H M R Mk |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 62 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | CDYL (E7S8T) Rabbit mAb recognizes endogenous levels of total CDYL protein. |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly255 of human CDYL protein. |
| Background | Chromodomain Y-like (CDYL) is a transcriptional corepressor and chromatin reader that recognizes the histone marks H3K9me3, H3K27me2, and H3K27me3, which are typically associated with repressive chromatin states and inhibition of gene expression. CDYL functions as a molecular bridge between the histone H3K9 methyltransferase G9a/EHMT2 and the transcription repressor REST, targeting G9a to the loci of REST target genes (1,2). CDYL has also been shown to directly interact with EZH2, the catalytic subunit of the Polycomb Repressive Complex 2 (PRC2), where it recruits EZH2 to H3K27me3 sites and propagates this repressive modification along chromatin (3). CDYL binds to methylated histone lysine residues through its N-terminal chromodomain, while its C-terminal enoyl-coenzyme A (CoA) hydratase-isomerase catalytic domain may be involved in histone lysine crotonylation (4). Knockout of CDYL is largely embryonic lethal in mice, with few mice surviving and dying shortly after birth (5). Additional studies in mice show that knockdown of CDYL appears to disrupt normal neuronal migration and brain development, possibly due to the loss of RhoA transcriptional repression (6). |
| SKU | CSIG-18764S |
|---|---|
| Featured | No |
| Supplier Part Number | 18764S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |