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Cell Signaling Phospho-Cd28 (Tyr191) (E5b9z) Rabbit mAb
List Price
$405.01
Your Price
$405.01
Cell Signaling Phospho-Cd28 (Tyr191) (E5b9z) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-16399S
MFG.PART:
16399S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H M |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:200} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-CD28 (Tyr191) (E5B9Z) Rabbit mAb recognizes endogenous levels of CD28 protein only when phosphorylated at Tyr191. This antibody shows cross-reactivity with phosphorylated EGFR, ERBB2, and CSF1R, and detects a 70-80 kDa band of unknown origin in some treated cell lines. |
| Species Reactivity | Human, Mouse |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr191 of human CD28 protein. |
| Background | CD28 is a transmembrane glycoprotein expressed by T cells as well as some other hematopoietic cells (1, 2). T cell activation requires T cell receptor (TCR) recognition of antigen presented in the context of MHC molecules. CD28 acts as a T cell costimulatory receptor, and interaction of CD28 with its ligands CD80 or CD86 provides the second signal required for naïve T cell activation (3-5). Activation of naïve T cells in the absence of CD28 stimulation can result in a state of T cell anergy, or unresponsiveness (3). CD28 signals through cytoplasmic phospho-tyrosine motifs that bind several SH2 or SH3 domain-containing proteins involved in T cell activation (2). Recently, CD28 was demonstrated to be a preferred target of PD-1-mediated dephosphorylation. Consistently, CD28 expression was required for T cell proliferation following PD-1 blockade and CD28 stimulation was required for effective anti-PD-1 cancer immunotherapy in mice (6, 7). Several CD28 isoforms are produced by alternative splicing (8). |
| SKU | CSIG-16399S |
|---|---|
| Featured | No |
| Supplier Part Number | 16399S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |