Cell Signaling Ubiquityl-Pcna (Lys164) (D5c7p) Rabbit mAb

Name: Cell Signaling Ubiquityl-Pcna (Lys164) (D5c7p) Rabbit mAb
SKU: CSIG-13439S
Price: 405.01 USD

List Price $405.01 Your Price $405.01

Cell Signaling Ubiquityl-Pcna (Lys164) (D5c7p) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-13439S

MFG.PART: 13439S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	44
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb recognizes endogenous levels of PCNA protein only when ubiquitinated at Lys164.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys164 of human PCNA protein.
Background	Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation.PCNA is mono-ubiquitinated at Lys164 in response to DNA damage at stalled replication forks. This modification is important in the translesion synthesis (TLS) DNA repair pathway (reviewed in 4,5).

Specifications