Cell Signaling Phospho-Gab1 (Tyr659) Antibody

Name: Cell Signaling Phospho-Gab1 (Tyr659) Antibody
SKU: CSIG-12745S
Price: 360.57 USD

List Price $360.57 Your Price $360.57

Cell Signaling Phospho-Gab1 (Tyr659) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-12745S

MFG.PART: 12745S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	110
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phosho-Gab1 (Tyr659) Antibody recognizes endogenous levels of Gab1 protein only when phosphorylated at Tyr659.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr659 of human Gab1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9).

Specifications