Cell Signaling Lin28a (D1a1a) Xp Rabbit mAb (Alexa Fluor 488 Conjugate)

Name: Cell Signaling Lin28a (D1a1a) Xp Rabbit mAb (Alexa Fluor 488 Conjugate)
SKU: CSIG-12573S
Price: 454.50 USD

List Price $454.50 Your Price $454.50

Cell Signaling Lin28a (D1a1a) Xp Rabbit mAb (Alexa Fluor 488 Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-12573S

MFG.PART: 12573S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl (50 tests)
Reactivity	H M
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Immunofluorescence (Immunocytochemistry): 1:400, Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	LIN28A (D1A1A) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total LIN28A protein.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro201 of human LIN28A protein.
Background	LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).

Specifications