Cell Signaling Beta-Catenin (D10a8) Xp Rabbit mAb (Sepharose Bead Conjugate)

Name: Cell Signaling Beta-Catenin (D10a8) Xp Rabbit mAb (Sepharose Bead Conjugate)
SKU: CSIG-12475S
Price: 456.52 USD

List Price $456.52 Your Price $456.52

Cell Signaling Beta-Catenin (D10a8) Xp Rabbit mAb (Sepharose Bead Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-12475S

MFG.PART: 12475S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	400 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	92
Source/Isotype	Rabbit IgG
Application/Dilution	{Immunoprecipitation: 1:20}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Specificity/Sensitivity	β-Catenin (D10A8) XP® Rabbit mAb (Sepharose® Bead Conjugate) recognizes endogenous levels of total β-catenin protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro714 of human β-catenin protein
Background	β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

Specifications