Cell Signaling Upf1 (D15g6) Rabbit mAb

Name: Cell Signaling Upf1 (D15g6) Rabbit mAb
SKU: CSIG-12040S
Price: 342.39 USD

List Price $342.39 Your Price $342.39

Cell Signaling Upf1 (D15g6) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-12040S

MFG.PART: 12040S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	140
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:100 - 1:400, eCLIP: 1:200}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	UPF1 (D15G6) Rabbit mAb recognizes endogenous levels of total UPF1 protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UPF1 protein.
Background	UPF1 was identified as an active component in nonsense-mediated mRNA decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). UPF1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component of cytoplasmic P-bodies (3). UPF1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, UPF2 and UPF3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively (5).

Specifications