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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, R | WB, IHC, FC, IP, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF106 |
| Description | Anti-Viperin Antibody, clone MaP.VIP |
| Alternate Names |
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| Background Information | Virus inhibitory protein endoplasmic reticulum-associated interferon-inducible (Viperin) belongs to the RSAD2 family and is highly inducible by both type I and type II interferons, but little or no induction is observed in moncytic cell lines. Studies suggest that viperin is involved in host antiviral response, as viperin induction is observed from viral infections. Research also reveals that viperin inhibits influenza replication by disrupting its release from the plasmic membrane through the altering of plasma membrane fluidity by affecting formation of lipid rafts. Lipid rafts are known detergent-resistant microdomian sites for influenza budding. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Viperin Antibody, clone MaP.VIP detects level of Viperin & has been published & validated for use in WB, IH, FC, IP & IC. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:500-1:1,000 dilution from a representative lot detected Viperin in human colon and rat liver tissues. Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HepG2 cell lysate (Hinson, E., et al. (2008). JBC. 284(7):4705–4712). Flow Cytometry Analysis: A representative lot was used by an independent laboratory in mouse liver, spleen, lung, and lymph node cells (Hinson, E., et al. (2010). J. Immunol. 184:5723-5731). Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in mouse spleen cells (Hinson, E., et al. (2010). J. Immunol. 184:5723-5731). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human Viperin. |
| Clone | MaP.VIP |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Involved in antiviral defense. May impair virus budding by disrupting lipid rafts at the plasma membrane, a feature which is essential for the budding process of many viruses. Acts through binding with and inactivating FPPS, an enzyme involved in synthesis of cholesterol, farnesylated and geranylated proteins, ubiquinones dolichol and heme. Plays a major role in the cell antiviral state induced by type I and type II interferon. Displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses, but not vesiculovirus. COFACTOR: Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine (By similarity). SUBUNIT STRUCTURE: Interacts with FPPS. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus. Note: Probably associates with the cytosolic side of the endoplasmic reticulum. Infection with human cytomegalovirus (HCMV) causes relocation to the Golgi apparatus and to cytoplasmic vacuoles which also contain HCMV proteins glycoprotein B and pp28. INDUCTION: By interferon type I, type II and bacterial lipopolysaccharides (LPS). Little or no induction by IFNG/IFN-gamma is observed in monocytic cell lines. Induced by infection with human cytomegalovirus (HMCV), hepatitis C virus, yellow fever virus and Sendai virus, presumably through type I interferon pathway. MISCELLANEOUS: Up-regulated in atherosclerosis. Latent viruses like HCMV may be involved in atherogenesis by initiating local inflammation. This may induce up-regulation of antiviral gene RSAD2, which modulates lipids synthesis, and thus could play a role in abnormal lipid accumulation leading to atherosclerosis. SEQUENCE SIMILARITIES: Belongs to the RSAD2 family. |
| Molecular Weight | ~42 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse macrophage stimulated with IFN gamma and LPS. Western Blot Analysis: 1 µg/mL of this antibody detected Viperin in 10 µg of mouse macrophage stimulated with IFN gamma and LPS. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |