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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2226 |
| Description | Anti-Piccolo Antibody, clone 4G3.3 |
| Alternate Names |
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| Background Information | Piccolo (presynaptic cytomatrix protein or PCLO) may act as a scaffolding protein involved in the organization of synaptic active zones. Synaptic vesicles dock and fuse in the active zone of the plasma membrane at chemical synapses. The presynaptic cytoskeletal matrix (PCM), which is associated with the active zone and is situated between synaptic vesicles, is thought to be involved in maintaining the neurotransmitter release site in register with the postsynaptic reception apparatus. The cycling of synaptic vesicles is a multistep process involving a number of proteins. Among the components of the PCM that orchestrate these events are Bassoon (BSN), RIM (RBBP8), Oboe, and Piccolo (PCLO) [supplied by ENTREZGENE]. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgMκ in buffer containing PBS and 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Piccolo Antibody, clone 4G3.3 is validated for use in WB, IH(P) for the detection of Piccolo. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin): Representative images from a previous lot. Piccolo staining pattern/morphology on mouse brain tissue/cerebellum. Tissue was pretreated with citrate pH 6.0, antigen retrieval. A previous lot of the antibody was diluted to 1:300, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is localized to the synaptic junction of neurons in the granular layer and Purkinje cells(arrow). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein |
| Clone | 4G3.3 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes piccolo. |
| Isotype | IgMκ |
| Species Reactivity |
|
| Species Reactivity Note | Mouse and Rat. Predicted to react with human based on 82% sequence homology and rat based on 92% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking (By similarity). SUBUNIT: Interacts with Rabac1/Pra1, RIMS2 and profilin (By similarity). SUBCELLULAR LOCATION: Cell junction, synapse (By similarity). Note=Concentrated at the presynaptic side of synaptic junctions (By similarity). ALTERNATIVE PRODUCTS: 3 named isoforms DOMAIN: C2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change. SIMILARITY: Contains 2 C2 domains. SIMILARITY: Contains 1 PDZ (DHR) domain. SEQUENCE CAUTION: Sequence=AAD21789.2; Type=Erroneous gene model prediction; |
| Molecular Weight | 547 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by western blot on rat brain lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |