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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS97 |
| Description | Anti-KEAP1 Antibody |
| Alternate Names |
|
| Background Information | Anti-oxidant defense is known to be regulated by various mechanisms including the usage of nuclear factor erythroid 2-related factor 2 (Nrf2). Under normal physiological conditions, Nrf2 is bound to Kelch-like ECH-associated protein 1 (Keap1), a cytoplasmic repressor. Keap1 also acts as a substrate adaptor for Cullin3-dependent ubiquitin ligase and also targets Nrf2 for degradation by proteosomes. The substrate adaptor function of Keap1 is inactivated during physiological stress. In this respective condition, a range of oxidative and electrophilic stimuli such as ROS, diethyl malonate, and certain disease processes, resulting from Nrf2 accumulation, enters the nucleus and thereby activates the expression of select anti-oxidant genes. Such genes promote the detoxification of ROS and other harmful molecules, which contribute to the carcinogenesis. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-KEAP1 Antibody detects level of KEAP1 & has been published & validated for use in WB & IC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected KEAP1 in A431 and HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the Kelch 3 region of human KEAP1. |
| Epitope | Kelch 3 Region |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the Kelch 3 region of KEAP1. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome. SUBUNIT STRUCTURE: Homodimer. Forms a ternary complex with NFE2L2 and PGAM5. Interacts with the N-terminal regulatory domain of NFE2L2/NRF2. Interacts with BPTF and PTMA. Interacts with CUL3. Part of a complex that contains KEAP1, CUL3 and RBX1. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between cytoplasm and nucleus. |
| Molecular Weight | ~60-70 kDa observed. A doublet may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected KEAP1 in 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |