Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M, Chp, Rhesus Macaque | IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS94 |
| Description | Anti-MLK1 Antibody |
| Alternate Names |
|
| Background Information | MLK1 (MAP3K9) is a serine–threonine kinase of the Mixed-Lineage Kinase (MLK) family capable of activating the c-Jun N-terminal Kinase (JNK)/Mitogen-Activated Protein Kinase (MAPK) pathway and regulating the other two prinicpal MAPK cascades, p38 and extracellular signal regulated kinase (ERK). Immunoblots and mass spectrometry show MLK is threonine phosphorylated in or near the activation loop. Studies show that MLKs play a role in the TGF-beta1-induced cell death mechanism. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MLK1 Antibody detects level of MLK1 & has been published & validated for use in IH(P). |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human MLK1. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Predicted to react with Rat, Mouse, Chimpanzee, and Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Activates the JUN N-terminal pathway. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Magnesium. ENZYME REGULATION: Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation (By similarity). SUBUNIT STRUCTURE: Homodimer (By similarity). TISSUE SPECIFICITY: Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin. PTM: Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. Contains 1 SH3 domain. SEQUENCE CAUTION: The sequence AAI11408.1 differs from that shown. Reason: Erroneous initiation. |
| Molecular Weight | 121 kDa (isoform 1), 123 kDa (isoform 2) calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in colorectal adenocarcinoma tissue. Immunohistochemistry Analysis: A 1:300 dilution of this antibody detected MLK1 in colorectal adenocarcinoma tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |