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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS222 |
| Replaces | 09-463 |
| Description | Anti-DEPTOR Antibody |
| Alternate Names |
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| Background Information | DEPTOR (DEPDC6, DEP domain-containing protein 6) was recently identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. DEPTOR negatively regulates mTORC1 and mTORC2. It has been shown that loss of DEPTOR activates various targets that include p70S6K1, Akt, and SGK1, that leads to cell growth and survival. Little was known about DEPDC6 prior to the discovery of its interactions with mTOR, at which time it was given the new name of DEPTOR (Peterson, T.R., et al. (2009). Cell. 137(5):873-876). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-DEPTOR Antibody detects level of DEPTOR & has been published & validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the DEP domain-containing mTOR-interacting protein of human DEPTOR. |
| Epitope | DEP domain-containing mTOR-interacting protein DEPTOR. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the DEP domain that contains mTOR-interacting protein DEPTOR. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Other homologies: Primate, Equine, Porcine, Canine, Rat, and Mouse based on 99%, 99%, 98%, 97%, and 96% homology, respectively. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes. SUBUNIT STRUCTUREInteracts (via PDZ domain) with MTOR; interacts with MTOR within both mammalian target of rapamycin complexes mTORC1 and mTORC2. |
| Molecular Weight | ~46 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HEK293 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected DEPTOR in HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |