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Achieve first time ChIP success with EZ-ChIP Assays
| Description | |
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| Catalogue Number | 16-663 |
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| Description | Magna ChIP™ Protein A+G Magnetic Beads |
| Overview | Recombinant protein A plus recombinant protein G covalently bound to magnetic beads. This magnetic A+G blend allows for the use of a wider range of antibodies than A or G alone and provides a rapid, reproducible and efficient reagent for collecting immunocomplexes for chromatin immunoprecipitation (ChIP) assays as well as RNA immunoprecipitation (RIP) assays. Compared with conventional agarose beads, magnetic beads significantly reduce handling times and mechanical stress on target immunocomplexes. |
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| Background Information | Chromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. Experiments comparing protein A vs. protein G vs. protein A/G magnetic bead blends revealed that a mixture of protein A and G beads worked well with a wide variety of antibody isotypes. The use of Protein A/G bead blends eliminated the need to consider which beads or kit to use in order match a particular antibody/bead binding affinity combination. In addition to simplifying the procedure, comparing the use of either protein A or protein G alone, protein A/G magnetic bead blends improved signal-to-noise ratios without decreasing the recovery of input chromatin in ChIP assays. |
| Product Information | |
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| Presentation | Liquid suspension. Supplied as magnetic bead slurry in phosphate buffered saline, pH 7.4, containing 0.01% Tween®-20 and 0.09% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
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| Application | This blend of protein A+G magnetic beads allows for the use of a wider range of antibodies than A or G alone & provides a rapid, reproducible & efficient collection of immunocomplexes for chromatin immunoprecipitations (ChIP) and RNA immunoprecipitations (RIP) assays |
| Application Notes | Use 20 µL of bead suspension per ChIP application. Includes sufficient reagents for 50 precipitation reactions. Disperse beads thoroughly before pipetting by rapid vortex. |
| Biological Information | |
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| Analytes Available |
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| Dimensions | |
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| Particle Size | ~3 µm |
| Volume | 1 mL |
| Product Usage Statements | |
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| Quality Assurance | Routinely evaluated by Chromatin immunoprecipitation (ChIP) using HeLa nuclear extracts and the Magna ChIP™ A Kit (Cat. #17-610). |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of shipment. Do Not Freeze. |
| Storage Temperature | 2 to 8 °C (Do not freeze) |
| Packaging Information | |
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| Material Size | 50 reactions |