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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IHC | Gt | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-1385 |
| Description | Anti-PDX1 (goat) Antibody |
| Alternate Names |
|
| Background Information | Transcription factor PDX1, also known as pancreatic and duodenal homeobox 1, activates expression of the insulin and somatostatin genes. This protein is a key regulator of islet peptide hormone expression and also plays an essential role in pancreatic development. It has been shown to interact with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). This protein also interacts with the methyltransferase SETD7. Mutations in this gene may be involved in several disorders of the pancreas or in diabetes mellitus. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Unpurified goat polyclonal serum containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PDX1 (goat) Antibody is a Goat Polyclonal Antibody for detection of PDX1 also known as pancreatic & duodenal homeobox 1, somatostatin transcription factor 1, Insulin promoter factor 1 & has been validated in WB & IHC. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: 1:400 dilution from a represenative lot detected PDX1 in normal human pancreas tissue. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to the N-terminus of mouse PDX1. |
| Epitope | N-terminus |
| Host | Goat |
| Specificity | This antibody recognizes the N-terminus of PDX1. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. Binds the DNA sequence 5-CC[CT]TAATGGG-3. SUBUNIT STRUCTURE: Interacts with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). Interacts with the methyltransferase SETD7 By similarity. Interacts with SPOP. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). DEVELOPMENTAL STAGE: At 8.5 dpc, detected in the gut epithelium from which the pancreatic buds are formed. Transient expression in pancreatic ducts, endocrine and acinar cells. Down-regulated around 10.5 dpc when expression becomes restricted to differentiated beta-cells. DOMAIN: The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter (By Similarity). The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y) (By Similarity). PTM: Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration. SEQUENCE SIMILARITIES: Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily. Contains 1 homeobox DNA-binding domain. |
| Molecular Weight | ~ 39 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse pancreatic β T-cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected PDX1 on 10 µg of mouse pancreatic β T-cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |