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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, R, M | WB, IP | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-1310 |
| Description | Anti-JAK2 Antibody |
| Alternate Names |
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| Background Information | The Janus family of tyrosine kinases (JAK) includes JAK1, JAK2, JAK3 and Tyk2. This family associates with a variety of cytokine receptors and is activated by ligand binding. Upon ligand binding to cytokine receptors, JAKs phosphorylate themselves and their associated receptors. This provides multiple binding sites for signaling proteins containing SH2 or other phospho-tyrosine-binding domains, including STATs, Shc, insulin receptor substrates and FAK. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-JAK2 Antibody is validated for use in WB, IP for the detection of JAK2. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 1 µg/mL from a previous lot detected JAK2 in 10 µg of L6 cell lysate. Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JAK2 from RAW264.7 cell lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the protein kinase 1 domain of mouse JAK2. |
| Epitope | Protein kinase 1 domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the protein kinase 1 domain of JAK2. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Tyrosine kinase of the non-receptor type, involved in interleukin-3 and probably interleukin-23 signal transduction (By similarity). Plays a role in leptin signaling and control of body weight. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT STRUCTURE: Interacts with IL23R, SKB1 and STAM2 (By similarity). Interacts with SIRPA and SH2B1. SUBCELLULAR LOCATION: Endomembrane system; Peripheral membrane protein By similarity. Note: Wholly intracellular, possibly membrane associated (By similarity). DOMAIN: Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1. PTM: Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. Contains 1 SH2 domain. |
| Molecular Weight | ~130 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in RAW264.7 cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected JAK2 in 10 µg of RAW264.7 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |