Cell Signaling Rif1 (D2f2m) Rabbit mAb

Name: Cell Signaling Rif1 (D2f2m) Rabbit mAb
SKU: CSIG-95558S
Price: 342.39 USD

List Price $342.39 Your Price $342.39

Cell Signaling Rif1 (D2f2m) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-95558S

MFG.PART: 95558S

UNSPSC: 41116111

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	274
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	RIF1 (D2F2M) Rabbit mAb recognizes endogenous levels of total RIF1 protein.
Species Reactivity	Human, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala400 of human RIF1 protein.
Background	The Rap1 interacting factor 1 (RIF1) was originally identified as a regulator of telomere homeostasis in yeast and mammalian cells (1). Research studies show that RIF1 regulates the timing of eukaryotic DNA replication origin firing through its effect on chromatin architecture (2-4). Additional studies show that RIF1 is essential for regulating the repair of DNA double strand breaks (DSBs). RIF1 is recruited to sites of DSBs by 53BP1 in response to DNA damage, and suppresses 5 end resection to favor the non-homologous end joining (NHEJ) pathway over homologous recombination (HR) repair (5-8). Oct-4 and Smad3 modulate Rif1 expression in mouse embryonic stem cells; Rif1 may regulate embryonic stem cell stability during cell proliferation (9).

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