Cell Signaling Phospho-Numa (Thr2055) Antibody

Name: Cell Signaling Phospho-Numa (Thr2055) Antibody
SKU: CSIG-14951S
Price: 360.57 USD

List Price $360.57 Your Price $360.57

Cell Signaling Phospho-Numa (Thr2055) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-14951S

MFG.PART: 14951S

UNSPSC: 41116111

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	238
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-NuMA (Thr2055) Antibody recognizes endogenous levels of NuMA protein only when phosphorylated at Thr2055.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr2055 of human NuMA protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The nuclear mitotic apparatus protein (NuMA) is a coiled coil protein involved in the formation and maintenance of the mitotic spindle. NuMA plays a role in chromatin organization during interphase, which influences mammary epithelial differentiation (1,2). During apoptosis, carboxy-terminal cleavage of NuMA may amplify signaling in the cell death pathway (2). NuMA is phosphorylated at numerous sites, with phosphorylation at Ser395 occurring in an ATM/ATR-dependent manner in response to DNA damage (3,4).Phosphorylation at Thr2055 by CDK1 is required for spindle pole association of NuMA at the onset of mitosis. Dephosphorylation by PPP2CA leads to enhancement of NuMA at the cell cortex in anaphase and proper cell-cycle progression (5,6).

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