Quality Bio Trypsin 2.5% w/v 100ml

Description

Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

• Without EDTA
• Without Phenol Red
• Without Calcium and Magnesium
• In PBS (Phosphate Buffered Saline)

Applications

Trypsin is a serine protease derived from porcine pancreas. It is a single chain polypeptide of 223 amino acid residue with substrate specificity based on positively charged Lysine and Arginine side chains. Trypsin predominantly cleaves peptide chains at the carboxyl sides of Lysine and Arginine, except when either is followed by Proline. It is most commonly used for dissociation and disaggregation of adherent cells.

Recommendation

Storage Conditions: -10°C to -20°C*

Shipping Condition: on dry ice

Sterile Filtered

Performance Tested

Max Shelf Life: 24 months from date of manufacture

Harmonization Code: 3822.00.5090

ECCN: EAR99

*NOTE: During shipping, the trypsin might change color from pink to yellow due to exposure to carbon dioxide released from the dry ice in the shipping container. In our experience, this discoloration does not effect the functionality of the trypsin.