Quality Bio QBSF-60 Serum Free Medium 500ml

Description

QBSF-60 is a proprietary serum-free, xeno-free expansion medium that supports the expansion of CD34+ human hematopoietic stem/progenitor cells.

QBSF-60 supports CD34+ cells derived from normal bone marrow, cord blood, peripheral blood stem cells mobilized with cytokines for ex-vivo expansion and differentiation to erythrocytes, granulocytes, and lymphocytes.

• Serum-Free, Xeno-Free Medium
• High cytokine-driven expansion rate fo CD34+ hematopoietic stem cells
• Contains L-Glutamine and Phenol Red
• Endotoxin, mycoplasma, and performance tested
• Sterile Filtered

Applications

QBSF-60 can be used for the maintenance of human hematopoietic stem cell culture, to support CD34 cell proliferation and differentiation to multi-lineage cells, and to support human umbilical and blood vessel endothelial cells

The QBSF products have been used successfully in different cell culture applications; investigators are encouraged to explore new uses.

QBSF products used in various applications

• Acute Sensitivity of Ph-like Acute Lymphoblastic Leukemia to the SMAC-Mimetic Birinapant. Richmond et al., Cancer Res. 2016 Aug 1;76(15):4579-91.
• Efficient iPS cell generation from blood using episomes and HDAC inhibitors. Hubbard JJ. et al., J Vis Exp. 2014 Oct 28;(92):e52009.
• Initial CD34+ cell-enrichment of cord blood determines hematopoietic stem/progenitor cell yield upon ex vivo expansion. Andrade et al., J Cell Biochem. 2011 Jul;112(7):1822-31.
• HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Yu et al., Stem Cells. 2006 Apr;24(4):876-88.
• Short-term exposure of umbilical cord blood CD34+ cells to granulocyte-macrophage colony-stimulating factor early in culture improves ex vivo expansion of neutrophils. Marturana et al., Cytotherapy. 2011 Mar;13(3):366-77.
• Stromal cell-free conditions favorable for human B lymphopoiesis in culture. Ichii et al., J Immunol Methods. 2010 Jul 31;359(1-2):47-55.
• Constitutive activation of STAT5A promotes human hematopoietic stem cell self-renewal and erythroid differentiation. Schuringa et al., J Exp Med. 2004 Sep 6;200(5):623-35.
• Ex Vivo Expansion of CD34+ Umbilical Cord Blood Cells in a Defined Serum-Free Medium (QBSF-60) with Early Effect Cytokines. Qiu et al., J. Hematotherapy & Stem Cell Research, 2004 Jul 9; 8(6):609
• Evaluation of Serum-Free Culture Conditions Able to Support the Ex Vivo Expansion and Engraftment of Human Hematopoietic Stem Cells in the Human-to-Sheep Xenograft Model, Almeida-Porada et al., J. Hematotherapy & Stem Cell Research, 2024, Jul 9; 9(5):591
• Support the Ex Vivo Expansion and Engraftment of Human Hematopoietic Stem Cells in the Human-t0-Sheep Xenograft Model, Almeida-Porada et al., J. of Hematotherapy & Stem Cell Research, 2024 July 9; 9(5):683
• Grafts of adenosine-releasing cells suppress seizures in kindling epilepsy. Huber et al. Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7611-6.
• Cytosine deaminase adenoviral vector and 5-fluorocytosine selectively reduce breast cancer cells 1 million-fold when they contaminate hematopoietic cells: a potential purging method for autologous transplantation. Garcia-Sanchez et al. Blood. 1998 Jul 15;92(2):672-82.
• Adaptation of BHK cells producing a recombinant protein to serum-free media and protein-free medium. Cruz et al. Cytotechnology. 1998 Jan;26(1):59-64.
• Serum-free culture conditions for cells capable of producing long-term survival in lethally irradiated mice. Brown et al. Stem Cells. 1997;15(3):237-45.

Storage Conditions: 2°C to 8°C

Shipping Condition: ambient temperature

Sterile Filtered

pH: 7.0± 0.2

Max Shelf Life: 24 months from date of manufacture

Harmonization Code: 3821.00.0000

ECCN: EAR99