Cayman sPLA2 (Type X) Inhibitor Screening Assay Kit

Cayman’s sPLA₂ (Type X) Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human sPLA₂ (Type X), a phospholipase that hydrolyzes PE and PC at the sn-2 position. The assay uses a sulfur-containing analog of 1,2-diheptanoyl-sn-glycero-3-PC (di-C7-PC) as a substrate. Upon hydrolysis by sPLA₂ (Type X) at the sn-2 position, free thiols can be detected by the sPLA₂ Assay Probe resulting in a product that can be easily quantified using its absorbance at a wavelength of 410 nm. The potent and reversible sPLA₂ (Type X) inhibitor, sPLA₂ Control Inhibitor, also known as LY315920 or varespladib, is included as a positive control and inhibits the enzyme with an IC₅₀ value of approximately 11 nM.

Needed but not supplied:Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.