Cayman Prostaglandin E Metabolite Elisa Kit; Size- 96 Solid Wells

Prostaglandin E₂ (PGE₂) is produced by a variety of cell types which, in general, do not contain the enzymes required for metabolism of PGE₂. Thus, cultured endothelial cells or osteoblasts will release PGE₂ into the culture medium, where it will accumulate without appreciable metabolism. The direct assay of PGE₂ from the medium is a good way to measure PGE₂ production from these cells. PGE₂ is rapidly converted in vivo to its 13,14-dihydro-15-keto metabolite, with more than 90% of circulating PGE₂ cleared by a single passage through the lungs. Unfortunately, this metabolite is not chemically stable and undergoes a variable amount of degradation to PGA products. For this reason, blood, urine, or other samples from whole animals or humans often contain very little intact PGE₂, and measurement of the metabolites is necessary to provide a reliable estimate of actual PGE₂ production. Cayman’s PGE Metabolite assay was developed as a method of converting 13,14-dihydro-15-keto PGA₂ and 13,14-dihydro-15-keto PGE₂ to a single, stable derivative that could be easily quantified by ELISA. This assay is therefore the method of choice if the samples in question have undergone extensive metabolism prior to collection.

Needed but not supplied:Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.