Cell Signaling Lars Antibody

Name: Cell Signaling Lars Antibody
SKU: CSIG-13868S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Lars Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-13868S

MFG.PART: 13868S

UNSPSC: 41116113

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	134
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	LARS Antibody recognizes endogenous levels of total LARS protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LARS protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Leucyl-tRNA Synthetase (LARS) is a leucine sensor critical for the activation of mTORC1 (1). mTORC1 kinase complex is an important component in the regulation of cell growth (2,3). Its activity is modulated by energy levels, growth factors, and amino acids (4,5). The four related GTPases, RagA, RagB, RagC, and RagD, have been shown to interact with raptor in mTORC1 (2,3). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (2,3). LARS functions as a GTPase-activating protein (GAP) and interacts directly with RagD GTPase (1). The role of LARS in leucine sensing is not related to its tRNA charging activity (1).

Specifications