Bio-Monolith Protein A and Protein G

Affinity chromatography is one of the most effective ways to determinate the titer of monoclonal antibodies. Protein A and Protein G affinity chromatography columns absorb the IgG molecules onto the affinity resins which contain either immobilized protein A ligand or protein G ligand, then the remaining impurities and byproducts from the fermentation broth can be removed. Elution of the purified monoclonal antibody and quantification by comparing the peak area to a calibration curve allows rapid measurement of the protein concentration. ;Agilent Bio-Monolith Protein A and Protein G affinity columns are designed for analytical separation of all IgG subclasses (human and mouse). They deliver fast and accurate quantitation of mAb titer (sub 1 minute) and purification of small amounts of mAb for subsequent CQA analysis by another complementary technique, such as Aggregate Analysis or Charge Variant Analysis, and can easily be combined into a 2D-LC workflow.;They are compatible with HPLC and UHPLC systems, including the Agilent 1100, 1200, 1260, and 1290 Bio-inert Quaternary LC Systems.