Sigma-Aldrich Neuron Coating Solution (5 ML)

Thaw Neuron Coating Solution at room temperature. Pipette a sufficient amount of the Neuron Coating Solution to culture ware to cover the entire culture surface. Incubate the culture ware at 37^oC for at least one hour or overnight.

Aspirate the Neuron Coating Solution from the culture ware. Rinse the culture surface twice with sterile PBS prior to use to remove unbound Neuron Coating Solution.