Key Specifications Table
| Key Applications | NMWL | Volume | Minimum Final Concentrate Volume |
|---|---|---|---|
| PExt, Protein Purification | 3 KDa | 0.5 mL | 15 µL |
| Description | |
|---|---|
| Catalogue Number | UFC500396 |
| Trade Name |
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| Description | Amicon Ultra-0.5 Centrifugal Filter Unit |
| Background Information | AMICON ULTRA 0.5ML - 3KDa cutoff. Available in 4 pack sizes: UFC500308 - 8 pack UFC500324 - 24 pack UFC500396 - 96 pack UFC5003BK - 500 pack Concentrates 500 µL down to 15 µL Concentration factor 25 x to 30 x High sample recovery (greater than 90% of dilute starting solution) Fast processing time - 10 to 30 minutes Reverse spin capability provides consistent recoveries Applications: Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms Purification of macromolecular components found in tissue culture extracts or cell lysates Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC Desalting, buffer exchange and protein dialysis Material Supplied: The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample. |
| Product Information | |
|---|---|
| Filter Code | PL |
| Color Code | Clear |
| Filter Type | Ultrafiltration |
| NMWL | 3 KDa |
| Quality Level | MQ400 |
| Applications | |
|---|---|
| Application | Ultracel-3 regenerated cellulose membrane, 0.5 mL sample volume |
| Key Applications |
|
| Biological Information | |
|---|---|
| Sterility | Non-Sterile |
| Physicochemical Information | |
|---|---|
| Centrifugal Application | Protein Concentration and Desalting |
| Dimensions | |
|---|---|
| Length | 4.99 cm (1.96 in.) |
| Diameter | 10.8 mm |
| Filtration Area | 1 cm² |
| Minimum Final Concentrate Volume | 15 µL |
| Volume | 0.5 mL |
| Materials Information | |
|---|---|
| Chemistry |
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| Device Material |
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| Packaging Information | |
|---|---|
| Material Size | 96 |