Key Specifications Table
| Key Applications |
|---|
| Cult |
| Description | |
|---|---|
| Catalogue Number | SF001-100P |
| Replaces | SF001-100 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | ESGRO Complete PLUS Clonal Grade Medium, 100ml |
| Overview | ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium provided with a selective GSK3β inhibitor to enhance viability of mouse embryonic stem (ES) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for mouse ES cell expansion and growth at clonal density in serum-free conditions. The mouse ES cells should maintain germ line competency. |
| Product Information | |
|---|---|
| Components |
|
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | The ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium provided with a selective GSK3β inhibitor to enhance viability of mouse embryonic stem cells & increased maintenance of pluripotency in the absence of serum & feeder cells. |
| Key Applications |
|
| Application Notes | Suitable for ES cell derivation, transfection and selection, in addition to general cell passaging and growth. Media can be used on feeder dependent/independent ES cell lines. Important passaging information Use of one of the below listed cell dissociation solutions is imperative while passaging ES cells using ESGRO Complete products. Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures. Recommended products include: ESGRO Complete™ Accutase (Cat # SF006) ESGRO Complete™ Trypsin Solution (proprietary) (Cat # SF007) ESGRO Complete™ Enzyme-Free Dissociation Solution (proprietary) (Cat # SF009) |
| Biological Information | |
|---|---|
| Media Form | Liquid |
| Stem Cell Type |
|
| Product Usage Statements | |
|---|---|
| Quality Assurance | QC PROTOCOL: Low density plating assay: 1. Plate feeder independent early passage ES cells onto gelatin coated 10 cm plastic tissue culture dish at 1000 cells/10 cm dish in 10-20 mL of Clonal Grade Medium. 2. Change medium every 2-4 days. 3. Monitor for clonal growth with alkaline phosphatase stain. Colony formation should occur over 4-6 days. The majority of colonies should be alkaline phosphatase positive and show no signs of differentiation. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | • Upon receipt, store clonal grade medium and supplement in the dark at -20°C. Refer to lot expiration date on labels. • Thawed clonal grade medium should be stored in the dark at 4°C for one month. Do not refreeze. • Supplement may be thawed at room temperature, aliquoted and stored in the dark at 4°C for two months. • After supplement is added to clonal grade medium, store in the dark at 4°C for up to two weeks. Do not refreeze. |
| Packaging Information | |
|---|---|
| Material Size | 100 mL |