Key Specifications Table
| Species | Host Cells | Protein Target |
|---|---|---|
| Human | Chem-5 | Mu / OP3 / MOP / MOR |
| Description | |
|---|---|
| Catalogue Number | HTS101M |
| Trade Name |
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| Description | CHEMISCREEN™ MEMBRANE PREPARATION RECOMBINANT HUMAN µ (Mu) OPIOID RECEPTOR |
| Overview | Full-length human OPRM1 cDNA encoding Mu |
| Background Information | Opiates derived from the opium poppy, Papaver somniferum, have been used in for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, µ κ and δ that activate Gi/o to reduce intracellular cAMP levels. Most clinically used opioids function by activation of the µ opioid receptor (Dhawan et al., 1996). Chemicons µ opioid receptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of µ opioid receptor interactions with its ligands. The membrane preparations exhibit an EC50 of 1.6nM for DAMGO in a GTPγS binding assay. |
| Product Information | |
|---|---|
| Format | Membranes |
| Presentation | Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Human Mu / OP3 / MOP / MOR GPCR membrane preparation for GTPγS Binding & Radioligand Binding Assays. |
| Key Applications |
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| Application Notes | GTPγS Binding and Radioligand Binding Assay. |
| Biological Information | |
|---|---|
| Entrez Gene Number |
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| Gene Symbol |
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| GPCR Class | A |
| Host Cells | Chem-5 |
| Protein Target | Mu / OP3 / MOP / MOR |
| Target Sub-Family | Opioid |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P35372 # Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin. SIZE: 400 amino acids; 44779 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P35372 ## Belongs to the G-protein coupled receptor 1 family. |
| Incubation Conditions | ASSAY CONDITIONS: Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/0.5 μM GDP in a nonbinding 96-well plate. Unlabeled DAMGO added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to an FB filter plate (Millipore MAHF B1H) previously prewetted with water, and washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4. The plate is dried and counted. One vial contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific DAMGO-stimulated [35S]-GTPγS binding. The μ opioid receptor membrane preparation is expected to be functional in a radioligand binding assay; however, the end user will need to determine the optimal radiolabeled ligand for use with this product. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | EC50 in GTPγS binding assay by DAMGO: ~ 1.6 nM |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. |
| Packaging Information | |
|---|---|
| Material Size | 200 units |