Key Specifications Table
| Species | Host Cells | Protein Target |
|---|---|---|
| Human | Chem-2 | NTR1 / NTS1 |
| Description | |
|---|---|
| Catalogue Number | HTS034M |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | ChemiSCREEN™ NTR1 Membrane Preparation |
| Overview | Human NTR1 |
| Background Information | Neurotensin is a 13 amino acid peptide that induces hypothermia and mediates analgesia when administered centrally, and also mediates the behavioral effects of antipsychotic drugs in rodents (Kinkead and Nemeroff, 2004). In addition, several intestinal functions, such as contractility and inflammation, are mediated by neurotensin (Castagliuolo et al., 1999). The actions of neurotensin are mediated by two GPCRs, NTR1 (or NTS1) and NTR2 (or NTS2). NTR1 knockout mice are resistant to the hypothermic, analgesic and contractile effects of neurotensin, indicating that NTR1 is the primary mediator of these effects (Pettibone et al., 2002). Small molecule antagonists, including SR48692 and SR142948A, that are partially selective for NTR1 also interfere with the biological effects of neurotensin (Labbé-Jullié et al., 1998). Chemicons NTR1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of neurotensin/NTR1 interactions. The membrane preparations exhibit a Kd of 1.5 nM for [125I]-neurotensin. With 2.5 μg/well NTR1 Membrane Prep and 0.5 nM [125I]-neurotensin, a greater than 7-fold signal-to-background ratio is obtained. |
| Product Information | |
|---|---|
| Format | Membranes |
| Presentation | PACKAGING: Packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSAPackaging method: Membranes protein were adjusted to 0.5 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80oC. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Human NTR1 / NTS1 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding. |
| Key Applications |
|
| Application Notes | Radioligand binding assay, and GTPgammaS binding. |
| Biological Information | |
|---|---|
| Concentration | 0.5 mg/mL |
| Entrez Gene Number |
|
| Entrez Gene Summary | Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. |
| Gene Symbol |
|
| GPCR Class | A |
| Host Cells | Chem-2 |
| Protein Target | NTR1 / NTS1 |
| Target Sub-Family | Neurotensin |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P30989 # Receptor for the tridecapeptide neurotensin. It is associated with G proteins that activate a phosphatidylinositol- calcium second messenger system. SIZE: 418 amino acids; 46259 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P30989 ## Belongs to the G-protein coupled receptor 1 family. |
| Incubation Conditions | Binding buffer: 50mM HEPES, 5mM MgCl2, 1mM CaCl2, 0.2% BSA, pH to 7.4 and filter. Radioligand: [125I] Neurotensin (PerkinElmer#: NEX198) One vial contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 5-fold signal:background with 125I-labeled neurotensin at 0.5 nM. |
| Product Usage Statements |
|---|
| 5 µg/well | |
|---|---|
| Signal:Background | 47 |
| Specific Binding (cpm) | 17556 |
SPECIFICATIONS: 1 unit = 5 µg
Bmax: 12.9 pmol/mg
Kd: 1.5 nM Usage Statement
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. |
| Packaging Information | |
|---|---|
| Material Size | 200 assays |