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Millipore 500 Ml Bcip/Nbt Single Reagent, Blue/Purple, Alkaline Phosphatase Substrate (Slightly Soluble In Alcohol) For Western Blotting & Ihc. - Mill (Additional S&H or Hazmat Fees May Apply)
BCIP/NBT Single Reagent, Blue/purple, Alkaline Phosphatase Substrate (slightly soluble in alcohol)
Overview
The most sensitive alkaline phosphatase substrate available for blotting techniques and in situ hybridization chemistry is the 5-bromo-4-chloro-3-indolyl phosphate/p-nitro blue tetrazolium system (BCIP/NBT). Using proprietary production and stabilization procedures, CHEMICON has made available a unique BCIP/NBT reagent that is stable at room temperature and several times more sensitive than existing formulations. For localization of enzyme labeled probes following Western, Northern, Southern or dot blotting, the reagent is added directly to the membrane. Following a 10-15 minute incubation, the reaction is terminated by distilled water rinses. Longer incubations may be employed if necessary, but use of biotin/streptavidin-alkaline phosphatase signaling complexes will negate prolonged reaction times.
Product Information
HS Code
3822 19 90
Presentation
5-bromo-4-chloro-3-indolyl phosphate p-nitro blue tetrazolium chloride in a proprietary buffer and enhancer.
Color Code
Black
Applications
Application
500 ml BCIP/NBT Single Reagent, Blue/purple, Alkaline Phosphatase Substrate (slightly soluble in alcohol) for Western Blotting & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at room temperature or 2-8°C for up to one year.
