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Millipore Active Complex Of Four (4) Full Length, Recombinant Human Enzymes: Fbw7, Skp1, Cul1 & Rbx1. For Use In Enzyme Assays. Functions As An E3 Ligase In Ubiquitination Assays. - Mill (Additional S&H or Hazmat Fees May Apply)
N-terminal c-Myc, 6His-tagged, recombinant human Smad7 full length, expressed by baculovirus in Sf21 insect cells.
Alternate Names
SCFFbxw7
SCFhCdc4
Background Information
The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1, and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCFFbw7 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell division, growth and differentiation, including Cyclin E1, c-Myc, Notch and c-Jun. Mutations have been found in the Fbw7 subunit of SCFFbw7 in various types of human cancers, therefore Fbw7 is considered to be a potent tumour suppressor.
Product Information
Quality Level
MQ100
Applications
Application
Active complex of four (4) full length, recombinant human enzymes: Fbw7, Skp1, Cul1 & Rbx1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
Key Applications
Enzyme Assays
Biological Information
Specific Activity
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Cascade Component
E3
Entrez Gene Number
NM_033632 (Fbw7)
NM_170679 (Skp1)
NM_003592 (Cul1)
NM_014248 (Rbx1)
Purification Method
Purified using glutathione sepharose.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
