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Millipore Active, Full Length, Recombinant Human Ubch5a. N-Terminal Ha, 6his-Tagged. For Use In Enzyme Assays. Functions As An E2 Enzyme In Ubiquitination Assays. - Mill (Additional S&H or Hazmat Fees May Apply)
N-terminal c-Myc, 6His-tagged, recombinant human Smad7 full length, expressed by baculovirus in Sf21 insect cells.
Alternate Names
Ubiquitin-conjugating enzyme E2 D1
Ubiquitin-protein ligase D1
Ubiquitin-conjugating enzyme E2-17 kDa 1
UBC4/5 homolog
Stimulator of Fe transport (SFT)
UbcH5
UBE2D1
Background Information
Ubiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or an Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate. The UbcH5 family (UbcH5a, UbcH5b and UbcH5c) have been shown to be the most active class of E2 enzymes in cell extracts and are associated with the regulation of a number of transcription factors, including p53 and c-Myc.
Product Information
Quality Level
MQ100
Applications
Application
Active, full length, recombinant human UbcH5a. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
Key Applications
Enzyme Assays
Biological Information
Specific Activity
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Cascade Component
E2
Entrez Gene Number
NM_003338
Purification Method
Purified using immobilized metal affinity chromatography.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
