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Millipore Activated Complex Of Three (3) Full Length, Recombinant Human Proteins: P27, Cyclin E1, & Cdk2. Phosphorylated In Vitro Using Cdk2/Cyclin E1. For Use In Enzyme Assays. Behaves - Mill (Additional S&H or Hazmat Fees May Apply)
C-terminal cMyc and 6His-tagged, recombinant human Smac/DIABLO amino acids 56-end, expressed in E.coli
Alternate Names
p27kip1
Cyclin-dependent kinase inhibitor 1B
Kip1
Background Information
p27 is an important regulator of cell cycle progression, involved in G1 arrest. It is a potent inhibitor of cyclin E1- and cyclin A-CDK2 complexes and a positive regulator of cyclin D-dependent kinases such as CDK4. The degradation of this protein, which is triggered by its CDK-dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Decreased levels of p27 are found in various epithelial tumours originating from lung, breast, colon, ovary, oesophagus, thyroid and prostate. Defects in the gene encoding p27 (CDKN1B) are the cause of multiple endocrine neoplasia type 4 (MEN4) an inherited cancer of the thyroid.
Product Information
Quality Level
MQ100
Applications
Application
Activated complex of three (3) full length, recombinant human proteins: p27, Cyclin E1, & CDK2. Phosphorylated in vitro using CDK2/Cyclin E1. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.
Key Applications
Enzyme Assays
Biological Information
Specific Activity
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Cascade Component
Substrate
Entrez Gene Number
BC001971(p27)
NM_001798 (CDK2)
NM_001238 (Cyclin E1)
Purification Method
Purified using immobilized metal affinity chromatography.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
