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Millipore Active Complex Of Five (5) Full Length, Recombinant Human Enzymes: Skp1, Skp2, Cul1, Rbx1 & Cks1. For Use In Enzyme Assays. Functions As An E3 Ligase In Ubiquitination Assays. - Mill (Additional S&H or Hazmat Fees May Apply)
C-terminal cMyc and 6His-tagged, recombinant human Smac/DIABLO amino acids 56-end, expressed in E.coli
Alternate Names
SCFSkp2
Skp2/Cks1 complex
Background Information
The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1 and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCFSkp2 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. It specifically recognizes phosphorylated p27 and is involved in regulation of G1/S transition. Complex formation of SCFSkp2 with Cks1 is required for the degradation of p27. An increased level of Skp2 and reduced level of p27 is linked to poor prognosis in a number of cancers
Product Information
Quality Level
MQ100
Applications
Application
Active complex of five (5) full length, recombinant human enzymes: Skp1, Skp2, Cul1, Rbx1 & Cks1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
Key Applications
Enzyme Assays
Biological Information
Specific Activity
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Cascade Component
E3
Entrez Gene Number
NM_005983 (Skp2)
NM_170679 (Skp1)
NM_003592 (Cul1)
NM_014248 (Rbx1)
NM_001826 (Cks1)
Purification Method
Purified using immobilized metal affinity chromatography.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
