Key Specifications Table
| Key Applications |
|---|
| HDAC |
| Description | |
|---|---|
| Catalogue Number | 20-175 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | HDAC Assay Buffer, 5X |
| Product Information | |
|---|---|
| Presentation | 5X HDAc Assay Buffer: 50mM Tris-HCl, pH 8.0, 750mM NaCl and 50% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity & represses transcription when tethered to a promoter. |
| Key Applications |
|
| Application Notes | For use in the Histone Deacetylase Assay (HDAC) Kit. |
| Biological Information | |
|---|---|
| Entrez Gene Number |
|
| Entrez Gene Summary | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q9UQL6 # Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. SIZE: 1122 amino acids; 121992 Da SUBUNIT: Interacts with BCOR, HDAC7, HDAC9, CTBP1, MEF2C, NCOR2, NRIP1, PHB2 and a 14-3-3 chaperone protein. Interacts with JARID1B. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.TISSUE SPECIFICITY: Ubiquitous.DOMAIN:SwissProt: Q9UQL6 The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm (By similarity). PTM: Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. & Ubiquitinated. Polyubiquitination however does not lead to its degradation. SIMILARITY: Belongs to the histone deacetylase family. Type 2 subfamily. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 1 year at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 10 mL |