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Key Specifications Table
| Species Reactivity | Key Applications |
|---|---|
| H, M | WB, ChIP |
| Description | |
|---|---|
| Catalogue Number | 17-603 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | ChIPAb+ Estrogen Receptor α - ChIP Validated Antibody and Primer Set |
| Overview | Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. ERα antibody is functionally validated in the precipitation of ERα associated chromatin. The qPCR primers included flank the ERα binding site in human pS2 promoter. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
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| Presentation | Anti-ERα (mouse ascites). 1 vial containing 100 mL ascites. Store at -20°C. The ERα antibody is made against aa120-170 of bovine estrogen a. It can recognize human and mouse ERα. ChIP primers TFF1 (pS2). 1 vial containing 75 mL of 5 μM of each control primer specific for human TFF1 (pS2) promoter. Store at -20°C. FOR: CCG GCC ATC TCT CAC TAT GAA REV: CCT TCC CGC CAG GGT AAA TAC |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This ChIPAb+ Estrogen Receptor α -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers. |
| Key Applications |
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| Application Notes | Chromatin Immunoprecipitation: Sonicated chromatin, prepared from 3x106 MCF7 cells that are either estrogen starved or b-estradiol treated (100 nM, 45 min.) was subjected to chromatin immunoprecipitation using 4 mL anti-ERa and the Magna ChIP™ G (Cat.# 17-611) kit Standard Protocol. Successful enrichment of ER associated DNA fragments was verified by qPCR using ChIP Primers TFF1 (pS2) flanking the human TFF1 promoter that contains an ER binding site (Please see figures). Please refer to the EZ-Magna ChIP™ G (Cat.# 17-409) or EZ-ChIP™ (Cat.# 17-371) kit protocols for experimental details. Western Blot Analysis: MCF7cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-ERa (1:1000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures). |
| Biological Information | |
|---|---|
| Immunogen | The ERα antibody is made against aa 120-170 of bovine estrogen α. |
| Host | Mouse |
| Specificity | Estrogen Receptor α |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5 UTRs and use different promoters. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. SIZE: 595 amino acids; 66216 Da SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1. SUBCELLULAR LOCATION: Nucleus. DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity. SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain. MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites. |
| Molecular Weight | 66 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Chromatin immunoprecipitation: Sonicated chromatin prepared from 3x106 MCF7 cells that were treated with b-estradiol (100 nM, 45 min.) was subjected to chromatin immunoprecipitation using 4 mL anti-ERα and beads only control. Successful enrichment of ERα associated DNA fragments was verified by qPCR using ChIP Primers TFF1 (pS2) flanking the human TFF1 promoter that contains an ER binding site (Please see figures). Please refer to the EZ-Magna ChIP™ G (Cat.# 17- 409) or EZ-ChIP™ (Cat.# 17-371) kit protocols for experimental details. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 1 year at -20°C from date of shipment |
| Packaging Information | |
|---|---|
| Material Size | 25 assays |
| Material Package | 25 assays per kit. ~4 μL per chromatin immunoprecipitation. |