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Millipore Catch And Release V2.0 Reversible Immunoprecipitation System
Millipore Catch And Release V2.0 Reversible Immunoprecipitation System
List Price$797.94Your Price$797.94
Millipore Catch & Release V2.0 Reversible Immunoprecipitation System To Purify Both Native & Denatured Proteins Using A Variety Of Antibodies & Sample Types. Comes In A Package Of 5 Immunoprecipitat - Mill (Additional S&H or Hazmat Fees May Apply)
Catch and Release® v2.0 Reversible Immunoprecipitation System
Overview
Features & Benefits: Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample Convenient: spin-column format dramatically simplifies sample handling Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use: This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IPs, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Product Information
Components
Catch and Release® Wash Buffer, 10X (Cat.# 20-210)
Catch and Release® Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
Catch and Release® Denaturing Elution Buffer, 1X (Cat.# 20-284)
20-285
20-216
Catch and Release® Capture Tubes
Quality Level
MQ100
Applications
Application
Catch & Release v2.0 Reversible Immunoprecipitation System to purify both native & denatured proteins using a variety of antibodies & sample types. Comes in a package of 5 immunoprecipitations.
Key Applications
Immunoprecipitation
IP-Kinase Assay
Western Blotting
Application Notes
Catch and Release® v2.0 should not be used with proteins containing a 6-His tag.
Biological Information
Species Reactivity
All
Product Usage Statements
Quality Assurance
routinely evaluated by immunoprecipitation.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
All components to be stored at 4°C except the Catch and Release® Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.
