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Millipore Pureepi Chromatin Preparation And Optimization Kit
Millipore Pureepi Chromatin Preparation And Optimization Kit
List Price$280.00Your Price$280.00
Millipore The Pureepi Chromatin Preparation & Optimization Kit Provides A Complete Set Of Reagents Allowing The Preparation Of Multiple Chromatin Samples From Starting Materials Such - Mill (Additional S&H or Hazmat Fees May Apply)
PureEpi™ Chromatin Preparation and Optimization Kit
Overview
The PureEpi Chromatin Preparation and Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues. A detailed protocol is provided that includes examples of the many variables involved in making chromatin on multiple instruments and how they may affect ChIP results.
Features & Benefits
Complete set of materials for up to 12 chromatin preparations
Optimized streamlined protocol with only a single buffer for sonication, IP, or wash
Protocols for using cells or tissues
Compatible with Magna ChIP HiSens and EZ-Magna ChIP HiSens chromatin immunoprecipitation kits.
Compatible with ChIPAb+ validated antibody and primer sets
Materials Required but Not Delivered
Reagents
Cells, stimulated or treated as desired
Tissue culture supplies appropriate for your cultured cell experiment
1 X PBS
37% Formaldehyde
The following items are necessary for the recommended method of crosslink reversal and DNA purification of sample:
Phenol
Chloroform:Isoamyl Alcohol
Phase Lock tubes
5M NaCl
Glycogen (20 mg/mL)
100% Ethanol
70% Ethanol
DNA purification column may possibly be used to purify DNA if organic extraction is not possible
RNAse A (10 mg/mL)
Proteinase K (10 mg/mL)
TE Buffer (Nuclease Free)
DNase and RNase-free sterile H2O
Liquid Nitrogen
100 bp DNA Ladder
1kb DNA Ladder
Agarose gel
DNA 12000 reagents for BioAnalyzer (Agilent)
Equipment
Vortex mixer
Microfuge
Sonicator
Variable temperature water bath or incubator
Timer
Variable volume (5-1000 µL) pipettors + tips
Cell scraper
1.5 mL microfuge tubes
NanoDrop® (Thermo-Fisher) or equivalent small volume capable spectrophotometer
Dewar flask for liquid nitrogen
Microscope and cell counter (Scepter or hemocytometer)
BioAnalyzer (Agilent)
Product Information
Components
10X Glycine
Nuclei Isolation Buffer
SCW Buffer (Sonication/ChIP/Wash)
Crosslink Reversal Buffer
Protease Inhibitor Cocktail II
Presentation
Two boxes containing all necessary reagents to perform 12 chromatin preparations using 15 cm plates of cultured cells or tissue samples.
Quality Level
MQ100
Applications
Application
The PureEpi Chromatin Preparation & Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.
