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N-terminal 6×His-tagged Drosophila NAP1 expressed in E. coli.
Background Information
Nucleosome assembly proteins (NAP) are involved in moving histones into the nucleus, nucleosome assembly and chromatin fluidity. They affect the transcription of many genes.
Product Information
Format
Purified
Presentation
10 mM K-HEPES pH 7.6, 10 mM KCl, 50 mM NaCl, 0.1 mM EDTA, 10%(v/v) glycerol, 0.01% NP-40, 0.2 mM PMSF, 1mM DTT. Frozen solution.
Quality Level
MQ100
Applications
Application
Active N-terminal His6-tagged Drosophila NAP1 expressed in E. coli.
Biological Information
Purity
Varies with individual lot. Lot R0803D0002 has purity 90% by SDS-PAGE and Coomassie blue staining
Source
Expressed in E. coli
Specific Activity
Varies with lot. Lot # R0803D0002: 12.5 µg NAP1 has been successfully used for 1 chromatin assembly reaction with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410).
Entrez Gene Number
37798
Purification Method
Ni2+/NTA-agarose
UniProt Number
Q9W1G7
Molecular Weight
MW = 43 kDa.
Product Usage Statements
Quality Assurance
Activity is routinely tested with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 6 months at -80°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
