| Description | |
|---|---|
| Catalogue Number | 14-835 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Aurora B Protein, active, 10 µg |
| Overview | His-tagged, recombinant, human Aurora B, full length and N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, His-tagged, recombinant, human Aurora B, full length & N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | human Aurora B, human INCENP co-expressed by baculovirus in Sf21 insect cells. |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]).[supplied by OMIM] |
| Gene Symbol |
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| Protein Target | Aurora-B |
| Purification Method | Glutathione-agarose |
| Target Sub-Family | AGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q96GD4 # May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates Ser-10 and Ser-28 of histone H3 during mitosis. COFACTOR: Magnesium. SIZE: 344 amino acids; 39280 Da SUBUNIT: Interacts with TACC1. Associates with RACGAP1 during M phase. Component of the CPC at least composed of BIRC5/survivin CDCA8/borealin, INCENP and AURKB/Aurora-B. Interacts with CDCA1 and KNTC2. Interacts with EVI5. SUBCELLULAR LOCATION: Nucleus. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid- body.TISSUE SPECIFICITY: High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.DISEASE:SwissProt: Q96GD4 # Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis. SIMILARITY: SwissProt: Q96GD4 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | Aurora B = 40 kDa INCENP = 38 kDa |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
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| Material Size | 10 ug |