| Description | |
|---|---|
| Catalogue Number | 14-721 |
| Brand Family | Upstate |
| Trade Name |
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| Description | EGFR (T790M, L858R) Protein, active, 10 µg |
| Overview | N-terminal GST-tagged, recombinant human EGFR, amino acids 696end containing the mutations T790M and L858R |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal GST-tagged, recombinant human EGFR, amino acids 696-end containing the mutations T790M & L858R, for use in Enzyme Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed by baculovirus in Sf21 cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Gene Symbol |
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| Protein Target | EGFR |
| Purification Method | Glutathione-agarose |
| Target Sub-Family | TK |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist. SIZE: 1210 amino acids; 134277 Da SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers. PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through Lys-63, but linkage through Lys-48, Lys-11 and Lys-29 also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980]. SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain. MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation. |
| Molecular Weight | 85.8kDa |
| Product Usage Statements | |
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| Quality Assurance | routinely evaluated by phosphorylation of 250μM substrate peptide (GGMEDIYFEFMGGKKK) |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -70°C from date of shipment |
| Packaging Information | |
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| Material Size | 10 µg |